Materials Supplied - DPC IMMULITE 2000 IL-6 Manual De Instrucciones

materials derived from human blood were
tested and found nonreactive for syphilis;
for antibodies to HIV 1 and 2; for hepatitis
B surface antigen; and for antibodies to
hepatitis C.
Sodium azide, at concentrations less than
0.1 g/dL, has been added as a
preservative. On disposal, flush with large
volumes of water to prevent the buildup of
potentially explosive metal azides in lead
and copper plumbing.
Chemiluminescent Substrate: Avoid
contamination and exposure to direct
sunlight. (See insert.)
Water: Use distilled or deionized water.

Materials Supplied

Components are a matched set. Labels on
the inside box are needed for the assay.
IL-6 Bead Pack (L26P12)
With barcode. 200 beads, coated with
monoclonal murine anti-IL-6. Stable at 2–
8°C until expiration date.
L2K6P2: 1 pack.
IL-6 Reagent Wedge (L26PA2)
With barcode. 18.5 mL protein/buffer
matrix and 18.5 mL alkaline phosphatase
(bovine calf intestine) conjugated to
polyclonal sheep anti-IL-6 in buffer, with
preservative. Stable at 2–8°C until
expiration date.
L2K6P2: 1 wedge.
Before use, tear off the top of the label at
the perforations, without damaging the
barcode. Remove the foil seal from the top
of wedge; snap the sliding cover down into
the ramps on the reagent lid.
IL-6 Adjustors (L6PL, L6PH)
Two vials (Low and High) of lyophilized
IL-6 in a protein/buffer matrix, with
preservative. Reconstitute each vial with
3.0 mL distilled or deionized water. Mix by
gentle swirling or inversion until the
lyophilized material is fully dissolved.
Stable at 2–8°C for 3 days after
reconstitution, or for 6 months (aliquotted)
at –20°C.
L2K6P2: 1 set.
Before making an adjustment, place the
appropriate Aliquot Labels (supplied with
the kit) on test tubes so that the barcodes
can be read by the on-board reader.
IMMULITE 2000 IL-6 (PIL2K6P-8, 2005-04-05)
Kit Components
Supplied Separately
Multi-Diluent 2 (L2M2Z, L2M2Z4)
For the on-board dilution of patient
samples. One vial of concentrated (ready-
to-use), nonhuman protein/buffer matrix,
with preservative. Stable at 2–8°C for 30
days after opening, or for 6 months
(aliquotted) at –20°C.
L2M2Z: 25 mL. L2M2Z4: 55 mL.
Barcode labels are provided for use with
the diluent. Before use, place an
appropriate label on a 16 × 100 mm test
tube, so that the barcode can be read by
the on-board reader.
L2M2Z: 3 labels L2M2Z4: 5 labels
L2SUBM: Chemiluminescent Substrate
L2PWSM: Probe Wash
L2KPM: Probe Cleaning Kit
LRXT: Reaction Tubes (disposable)
L2ZT: 250 Sample Diluent Test Tubes
(16 × 100 mm)
L2ZC: 250 Sample Diluent Tube Caps
LILCM: A bi-level, human serum-based
IMMULITE Cytokine Control Module
containing IL-6.
Also Required
Distilled or deionized water; test tubes;
controls.
Assay Procedure
Note that for optimal performance, it is
important to perform all routine
maintenance procedures as defined in the
IMMULITE 2000 Operator's Manual.
See the IMMULITE 2000 Operator's
Manual for: preparation, setup, dilutions,
adjustment, assay and quality control
procedures.
Recommended Adjustment Interval:
2 weeks.
Quality Control Samples: Use controls
or sample pools with at least two levels
(low and high) of IL-6.
Expected Values
A reference range study for
IMMULITE 2000 IL-6 was performed on
serum samples collected from 60 healthy
laboratory volunteers into plain glass
serum tubes. The non-parametric lower
95% range was from nondetectable to
3