Sample Preparation - BÜHLMANN B-CALEX-C50 Instrucciones De Uso

ENGLISH

Sample Preparation

1 Hold the CALEX
white sampling pin by simultaneously turning and pull-
ing it upwards (picture A).
Caution: Do not unscrew the blue cap!
2 Dip the dosing tip with the grooves into the stool and
twist before removing. Repeat the procedure at 3-5 dif-
ferent positions of the stool sample in order to fill the
grooves completely (picture B).
Note: Excess stool is stripped off in the funnel during
re-introduction of the sampling pin into the CALEX
Cap body.
3
Re-introduce the sampling pin into the CALEX
body and push it back into the final locking position
(you feel and hear two clicks, picture C).
4 Vortex the CALEX
down vigorously on a vortex mixer for 30 sec (picture
D) and allow it to stand for 10 min on the blue cap. If
the stool does not completely come off the grooves,
repeat the procedure until the grooves are completely
free of stool. That is easily monitored by checking that
the grooves are empty (refer to page 4, fig. B). Before
proceeding with step 5, please refer to the instruction
for use of the corresponding BÜHLMANN assay for
specific processing time of samples and centrifu-
gation.
Note: The stool sample is now diluted 1:500.
5
To measure the sample, either place the CALEX
into a preanalytical system according to the
appropriate instructions or manually unscrew the blue
cap (picture E) to access the sample for further
analysis.
6
Tightly recap the CALEX
usage.
Note: Robotic systems will generally close tubes auto-
matically with an appropriate sealing.
B-CALEX-C
Cap device upright and remove the
®
Cap device with the white head
®
Cap device after manual
®
A
B
®
1
Cap
®
C
D
Cap
®
E
5
2
3
Release date: 29-04-2020