Instructions For Use; Start-Up - Terragene Bionova IC10/20FR Manual Del Usuario

Instructions for use

Start-up

All equipment is manufactured with the fo-
llowing settings:
- Time zone: UTC +0:00
- Printing language: English
For changing time zone, refer to Time zone change
mode section.
For changing printing language, refer to Printing lan-
guage section.
1-Place the auto-reader on a firm surface, free from
vibrations, away from sunlight, currents of hot or cold
air, chemicals and corrosive or flammable substances.
Do not place the equipment in a way that disconnec-
tion of the plug from the power supply could be diffi-
cult. Leave a gap of at least 10 cm from the wall. Do
not move the auto-reader periodically or during its
use. Connect the auto-reader to a secure and stable
electrical power source.
Do not wet or heat the auto-reader. If liquid is
spilled on the incubator, disconnect it and dry
it immediately. Before turning on, verify that all
reading positions are empty.
2-Turn on the machine by connecting the end of the
AC power supply to the mains and then connect the
other end of the source (plug) on the rear of the IC10/
20FR unit. The printer's paper indicator light will turn
on, if this light becomes intermittent, this means that
the thermal paper must be loaded, or that the printer
cover is not tightly closed. Otherwise, the light will
remain stationary.
The machine will start running the last time and tem-
perature set-up.
If the incubator has an out-of-date Firmware
version, the settings might not be saved.
3-Synchronize IC10/20FR with local time using Biono-
va® software available at
ar/productos/control-de-infecciones/control-de-pro-
cesos-de-esterilizacion/incubadoras/ic10-20fr/.
For this, connect a PC to the USB plug of the incubator
and start the program. The Bionova® IC10/20FR will be
synchronized with the time in the PC. Synchronization
can be done as long there is not an ongoing reading.
4- Check that the incubation temperature is correct.
To change it, press the
Lights corresponding to 37 °C and 60 °C will start blin-
king, indicating that a temperature program must be
selected. For temperature selection, press the
button to select 37 ºC or 60 ºC. After 4 seconds the
temperature modification will be accepted.
For 37 ºC, the IC10/20FR auto-reader will automati-
cally select the 4 hrs. final reading program in both
halves of the incubation area (1-6 and 7-12).
For 60 ºC, 30 min., 1, 2 or 3 hrs. program can be selec-
ted. It's possible to select different programs for each
half or the same for both, depending on the indica-
tor´s final reading characteristics.
http://www.terragene.com.
button for 3 seconds.
IC10/20FR Page 6
Check that the time setting is appropriate for the BIs
to be incubated in each zone.
To change the time program, press the
of the correspondent zone as many times as needed
to choose the right setting.
5- Wait for temperature of the machine to be stable.
Once is stable, the indicator of the desired temperatu-
re will remain steady.
Do not put any biological indicator in the rea-
ding positions until the incubator does not
confirm temperature stability.
The auto-reader will keep the selected incubation pro-
gram during the reading process. The system does not
allow to modify the incubation program once the rea-
ding process has begun, unless the reading is finished.
6-Start readings once the blue temperature selec-
tion's light remains stable, this indicates that incu-
bation temperature was reached. Once the selected
temperature has been reached and remains stable,
after turning on the equipment, a one-time automa-
tic check of the Biological Indicators and Protein Pen
incubation positions will be carried out. Indicating
lights of each well will turn on bringing the result, a
green light will show a satisfactory result while a red
light will indicate error.
When the equipment detects an error in any position,
a ticket indicating the day, time and position involved
will be printed. To guarantee the reliability of the re-
sults, these positions will be deactivated, thus preven-
ting their use. To evidence this, the red light of that
position will remain blinking indefinitely, in order to
indicate that the position has been disabled.
For a correct automatic check, the protective
cover of the incubator should be in its place.
Read BIs instructions for use. Before placing the
vials in the incubator, press the top to seal the
tube. Crush the ampoule (using the ampoule
crusher within the box of BIs or the one located
within the incubation area of the incubator)
and make sure that the liquid has completely
wet the spore carrier placed at the bottom of
the biological indicator plastic tube.
Do not remove or change placement of BI once
the reading process has begun. If this occurs,
results may be invalidated.
Use a non-sterilized biological indicator as a
positive control every time a processed indica-
tor is incubated. For more information refer to
the Positive Control section of this user guide.
7-Place the rapid BI in a reading position and wait for
the result. Reading will start automatically. Immediate-
ly after placing the BI in a position, an alarm will sound
indicating that reading has successfully began and the
position LED will blink until the result is informed.
button