Hydrolysis For Fat Determination - Selecta DQO 6 Manual De Instrucciones

INSTRUCTION MANUAL CODE 80158 REV D
INSTRUCTION MANUAL CODE 80158
DG-04
Material needed (for 6 samples):
• 6 glass funnels Ø100mm
• 6 filter papers Ø100 normal (not slow one)
• 6 digestion tubes (SELECTA code 4042300)
• Digestion Block + RAT + Fume collector (SELECTA codes 4000507 + 4000051 + 4005072)
• 6 Erlenmeyer 500ml.
Reagents needed:
• 1L. of HCl 4N (350ml de HCl 35% and complete until 1L. with distilled water)
• Granules of pumice stone.
Procedure:
• Number the digestion tubes.
• Weight around 3gr. of sample and put it into the digestion tube.
• Add 100 ml of HCl 4N in each tube.
• Add a spoonful of granules of pumice stone.
• Place this into the block and digest at 100ºC - 130ºC during 1 hour (see page 12 table). It m
• Retire the digestion block and let it cool down.
• Prepare the Erlenmeyer with the funnels and the filter paper. See the photo below.
• Number the Erlenmeyer.
• Empty the content of the sample tube into the funnel with filter paper.
• Clean the tube with water and empty into the funnel. Repeat it until the water from the tube
• Put water on the funnel.
• Empty the Erlenmeyers.
• Repeat the operation until the water from Erlenmeyer is transparent (about 5 times).
• Let completely dry the paper filter (1 hour).
• You will observe that the filter keeps the sample fat.
• Proceed to the extraction with the det-gras equipment. Let dry for 5h, and to extract in two
and rinsing and let dry for 30 minutes.
J
J
.P. SELECTA s.a.u.
.P . SELECTA s.a.
10

Hydrolysis for fat determination

Material: (for 6 samples)
• 6 glass funnels Ø100mm
• 6 filter papers Ø100 normal (not slow one)
• 6 digestion tubes (SELECTA code 4042300)
• Complete digestion system.
• 6 Erlenmeyer 500ml.
Reagents needed:
• 1L of HCl 4N (350ml de HCl 35% and add until 1L. with distilled water)
• Granules of pumice stone.
PREVIOUS HYDROLISIS FOR THE FAT DETERMINATION: Cheese, egg a
Procedure:
• Number the digestion tubes.
• Weight around 3gr. of sample and put it into the digestion tube.
• Add 100 ml of HCl 4N in each tube.
• Add a spoonful of granules of pumice stone.
• Place this into the block and digest at 100ºC - 130ºC during 1 hour
(see page 12 table). It must boil slowly.
• Retire the digestion block and let it cool down.
• Prepare the Erlenmeyer with the funnels and the filter paper. See the
photo below.
• Number the Erlenmeyer.
• Empty the content of the sample tube into the funnel with filter paper.
• Clean the tube with water and empty into the funnel. Repeat it until the
water from the tube is transparent.
• Put water on the funnel.
• Empty the Erlenmeyers.
• Repeat the operation until the water from Erlenmeyer is transparent
(about 5 times).
• Let completely dry the paper filter (1 hour).
• You will observe that the filter keeps the sample fat.
• Proceed to the extraction with the det-gras equipment. Let dry for 5h,
and to extract in two phases: Boiling and rinsing and let dry for 30
minutes.
E
STE ENSA YO REQUIRE LA MANIPULACIÓN DE SUSTANCIAS PELIGROSAS
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OCT/2019 (It can be modified without notice)
REV B 12/04/2011
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(It can be modified without notice)
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