Ise Calibration - Thermo Scientific Orion Star Manual Del Usario

ISE Technique

ISE Calibration

The calibration standards should be prepared in the same ISE units as the
desired sample results . Start the calibration with the lowest concentration
calibration standard and work up to the highest concentration calibration standard .
Any reagents, such as ionic strength adjustors, should be added to samples
and standards as specified in the electrode user guide .
1 . Prepare the electrode, standards and any other required solutions for use
according to the electrode user guide .
2 . In the measurement mode, press
until the ISE icon is shown and press
press
3 . Rinse the electrode with distilled or deionized water, shake any excess water off,
blot it dry and place the electrode into the least concentrated standard .
4 . Wait for ISE icon to stop flashing . Press
is flashing, press
continue to change the digits until the meter displays the concentration value
of the standard . Once the standard value is set, press
point is in the correct location .
5 . Press to proceed to the next lowest calibration standard and repeat
steps 3 and 4, working from the lowest concentration standard to the highest
concentration standard, or press
6 . The actual electrode slope, in mV per decade concentration, will be displayed
in the main field and
a . For a one point calibration, press
To change the sign of the slope to negative or positive, press
none of the digits are blinking and the arrow icon is blinking and press
to change the sign of the slope . Press
mode .
b . For a two or more point calibration, the meter will automatically proceed
to the measurement mode after the slope is displayed .
EN-46
until the arrow icon points to the top line,
/ to change the value of the flashing digit and
to save and end the calibration .
will be displayed in the lower field .
Thermo Scientific Orion Star
to begin the calibration .
until the first digit to be changed
and / to edit the slope .
to return to the measurement
and Star Plus Meter User Guide
™
until the decimal
until